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High Sensitivity Spectrograph for Use in Fluorescence Microscopy

✍ Scribed by Frederix, P. L. T. M.; Asselbergs, M. A. H.; van Sark, W. G. J. H. M.; van den Heuvel, D. J.; Hamelink, W.; de Beer, E. L.; Gerritsen, H. C.


Book ID
115364002
Publisher
Society for Applied Spectroscopy
Year
2001
Tongue
English
Weight
353 KB
Volume
55
Category
Article
ISSN
0003-7028

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The use of polystyrene fluorescent microspheres as sensitive labels in direct-detection (not enzymatically amplified) heterogeneous equilibrium "sandwich" immunoassays in 96-well plates is described. With mouse IgG as a model antigen, a fluorescent particulate label is more sensitive than a correspo