High sensitivity for color mutants in lacZ plasmid-based transgenic mice, as detected by positive selection
✍ Scribed by Michaël E.T.I. Boerrigter
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 202 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Transgenic mice carrying bacteriophage lambda-system using phenyl-b-galactoside. The proportion based vectors harboring a lacZ or lacI reporter of color mutants in brain and kidney was 30 -40%. gene have been used in recent years for the detec-This was significantly higher than the 10 -20% color tion, quantification, and characterization of sponta-mutants observed in lung, spleen, and liver. It is neous and induced gene mutations in vivo. The use-demonstrated that the color mutants detected were fulness of these models is basically determined by neither the result of a mixture of both mutant and nontheir ability to detect significant (organ-specific) mutant cells within the original mutant colony-forming exposure-related increases in mutant frequencies. unit nor due to the presence of mutant and nonmutant This, in its turn, is dependent on the models' ability lacZ genes within individual E. coli host cells. Seto detect all possible types of mutations, including quence analysis of 13 different color mutants revealed mutations that partially inactivate the gene product, single basepair substitutions in the lacZ gene of each as well as the absence of a significant proportion mutant. The high tolerance of the positive selection of E. coli-derived mutations. The newly developed system for lacZ color mutants in the plasmid-based lacZ plasmid-based transgenic mouse mutation transgenic mouse model greatly contributes to the senassay is sensitive to a broad range of DNA muta-sitivity of this model, now ranging from large sizetions, including large size changes. Here, we de-change mutations that completely inactivate the lacZ scribe the plasmid model's sensitivity for detecting gene to basepair substitutions that partly inactivate color mutants, i.e., cells containing a partially inacti-the lacZ gene.