High Sensitivity Collisionally-activated Decomposition Tandem Mass Spectrometry on a Novel Quadrupole/Orthogonal-acceleration Time-of-flight Mass Spectrometer

✍ Scribed by Howard R. Morris; Thanai Paxton; Anne Dell; Jean Langhorne; Matthias Berg; Robert S. Bordoli; John Hoyes; Robert H. Bateman

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 627 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19960610)10:8<889::aid-rcm615>3.0.co;2-f

✦ Synopsis

Consideration of the special pmblems encountend in ultra-high sensitivity biopolymer sequencing studies has led to the development of a novel quadrupde/orthogonal-aderation time-of-jlight tandem mass spectrometer dcscn'bed for the first time here. The performance characteristics of this new geometry are demonstrated, including fdly resolved daughter-ion spectra with mass accurades of 0.1 dalton, which allow removal of interpretation ambiguities d easy differentiation of charge states even in weak collisionally-activated decomposition tandem m a s spectra. The instrum ent has been applied to a variety of biopolymer reserurh problems, including the structure determination of mqior histocompatibfity complex peptide antigens using liquid c h r o m a ~p h y / e l h p r a y mass spectrometry and nanoflow-eleetrmpray tandem mass spectrometry, and sequencing capability in the low-femtomole and attomole ranges is demonstrated. CCC 09514198/961080889-08 8 19% by John Mley & Sons, Ltd.