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High sensitivity analysis of proteins and peptides by capillary electrophoresis-tandem mass spectrometry: Recent developments in technology and applications

✍ Scribed by Dr. Daniel Figeys; Ruedi Aebersold

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
1023 KB
Volume
19
Category
Article
ISSN
0173-0835

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✦ Synopsis

Analytical biochemistry, in particular the analysis of regulatory proteins that control biological systems and pathways, is dependent on methods of ever-increasing sensitivity. Capillary electrophoresis (CE) has long been recognized as an ultrasensitive analytical technique. In spite of the high sensitivity, CE has not penetrated protein discovery research as a standard analytical method. In this review article we summarize recent technical developments which have significantly enhanced CE as a tool for the analysis of trace amounts of proteins. Specifically, we review recent advances in the development and application of capillary electrophoresis-mass spectrometry (CE-MS) and on-line analyte concentration techniques, and introduce the emerging field of microfluidics as a front end to mass spectrometry (MS).

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