High Sensitive Electrochemical Detection of Sequence-Specific DNA Using Low Current Voltammetry

✍ Scribed by Lingling Lin; Jianzhang Zhou; Yan Zhang; Zhonghua Lin

• Book ID: 102184127
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 282 KB
• Volume: 20
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200804248

✦ Synopsis

Abstract

In this article, we report on efforts to construct a high sensitive electrochemical sensor with immobilized sandwich‐type DNA borne ferrocene (Fc) head for sequence‐specific DNA detection using ultramicroelectrode and low current voltammetry. Based on the difference in deformability between the bending rigid complementary DNA double helix and its anomalous flexile mismatches, the fully complementary target can be distinguished from mismatched targets including the single‐base mismatched target. Detection limit estimated as the amount of DNA is observed to be 100 fM via low current voltammetry. The method offers great promise of high sensitivity and selectivity simultaneously for effective gene identification.