High resolution tiling-path BAC array deletion mapping suggests commonly involved 3p21-p22 tumor suppressor genes in neuroblastoma and more frequent tumors
✍ Scribed by Jasmien Hoebeeck; Evi Michels; Björn Menten; Nadine Van Roy; Angelika Eggert; Alexander Schramm; Katleen De Preter; Nurten Yigit; Els De Smet; Anne De Paepe; Geneviève Laureys; Jo Vandesompele; Frank Speleman
- Book ID
- 102273756
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- French
- Weight
- 237 KB
- Volume
- 120
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
The recurrent loss of 3p segments in neuroblastoma suggests the implication of 1 or more tumor suppressor genes but thus far few efforts have been made to pinpoint their detailed chromosomal position. To achieve this goal, array‐based comparative genomic hybridization was performed on a panel of 23 neuroblastoma cell lines and 75 primary tumors using a tiling‐path bacterial artificial chromosome array for chromosome 3p. A total of 45 chromosome 3 losses were detected, including whole chromosome losses, large terminal deletions and interstitial deletions. The latter, observed in cell lines as well as a number of distal deletions detected in primary tumors, allowed us to demarcate 3 minimal regions of loss of 3.6 Mb [3p21.31‐p21.2, shortest regions of overlap (SRO)1], 1.4 Mb (3p22.3‐3p22.2, SRO2) and 3.8 Mb (3p25.3‐p25.1, SRO3) in size. The present data significantly extend previous findings and now firmly establish critical regions on 3p implicated in neuroblastoma. Interestingly, the 2 proximal regions coincide with previously defined SROs on 3p21.3 in more frequent tumors including lung and breast cancer. As such, similar tumor suppressor genes may play a critical role in development or progression of a variety of neoplasms, including neuroblastoma. © 2006 Wiley‐Liss, Inc.