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High-resolution solid-state MAS NMR of proteins—Crh as an example

✍ Scribed by Anja Böckmann

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
537 KB
Volume
45
Category
Article
ISSN
0749-1581

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✦ Synopsis

Solid-state NMR spectroscopy provides unique possibilities for the structural investigation of insoluble molecules at the atomic level. Recent efforts aim at solving the complete structures of biological macromolecules using high-resolution magic angle spinning NMR. Structurally homogenous samples of [(13)C,(15)N]-labeled proteins have to be used in this type of studies. Microcrystalline model proteins present valuable tools for the developments of methods towards this goal. This review discusses recent progress in the field, using the Crh protein as an illustrative example. We discuss strategies for resonance assignments and for the determination of structure and dynamics, as well as techniques for the detection of protein interaction partners and folding mechanisms by solid-state NMR methods.

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