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High-resolution scanning electron microscopy of the cells of mineralized tissues

✍ Scribed by Segawa, K. ;Debari, K. ;Sasaki, T. ;Takiguchi, R.

Publisher
Wiley (John Wiley & Sons)
Year
1985
Tongue
English
Weight
189 KB
Volume
2
Category
Article
ISSN
0741-0581

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✦ Synopsis

h c e n t l y high-resolution scanning e l e c t r o n microscopy (SEM) enploying t h e osmiundemethyl s u l f o x i d e (DMSO)osmium inethod (OD0 method) h a s provided three-dimensional views of membranous c u n p n e n t s o f cell o r g a n e l l e s . This new technique includes tw i m p r t a n t t i s s u e p r e p a r a t i o n s : freezecracking ( 1 ) or c r p -p l i s h i n g ( 2 ) of t i s s u e s an3 maceration and removal of cytoplasmic matrices with d i l u t e osmium. t h e modified 01x) method to d i s p l a y three-dimensional apperances o f cell o r g a n e l l e s frm such n i n e r a l i z e d tissues as bone and t e e t h . MTERIALS AND METriODS Newborn ( 5 -10 days o l d ) Wistar-strain r a t s were

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