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High-resolution magic angle spinning proton NMR analysis of human prostate tissue with slow spinning rates

✍ Scribed by Jennifer L. Taylor; Chin-Lee Wu; David Cory; R. Gilberto Gonzalez; Anthony Bielecki; Leo L. Cheng

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
373 KB
Volume
50
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The development of high‐resolution magic angle spinning (HR‐MAS) NMR spectroscopy for intact tissue analysis and the correlations between the measured tissue metabolites and disease pathologies have inspired investigations of slow‐spinning methodologies to maximize the protection of tissue pathology structures from HR‐MAS centrifuging damage. Spinning sidebands produced by slow‐rate spinning must be suppressed to prevent their complicating the spectral region of metabolites. Twenty‐two human prostatectomy samples were analyzed on a 14.1T spectrometer, with HR‐MAS spinning rates of 600 Hz, 700 Hz, and 3.0 kHz, a repetition time of 5 sec, and employing various rotor‐synchronized suppression methods, including DANTE, WATERGATE, TOSS, and PASS pulse sequences. Among them, DANTE, as the simplest scheme, has shown the most potential in suppression of tissue water signals and spinning sidebands, as well as in quantifying metabolic concentrations. Magn Reson Med 50:627–632, 2003. © 2003 Wiley‐Liss, Inc.

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