High-Resolution Imaging of Cellular Proteins: Methods and Protocols

✍ Scribed by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski

Publisher: Humana Press
Year: 2016
Tongue: English
Leaves: 381
Series: Methods in Molecular Biology 1474
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.

✦ Table of Contents

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Front Matter....Pages 3-24
Front Matter....Pages 25-47
Front Matter....Pages 49-59
Back Matter....Pages 61-71
....Pages 73-90

✦ Subjects

Imaging / Radiology

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