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High-resolution hydroxyapatite chromatography of proteins

✍ Scribed by Michael John; Jürgen Schmidt

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
525 KB
Volume
141
Category
Article
ISSN
0003-2697

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✦ Synopsis

Hydroxyapatite chromatography has been used to separate all five isozymes of lactic dehydrogenase, six enzymatically active forms of bovine pancreatic DNase I, and a standard protein mixture. The proteins were eluted with a linear gradient of sodium phosphate. Enzyme activity recoveries were greater than 90%. Packing materials were obtained from commercial fine-particle-sized hydroxyapatite (DNA grade Bio-Gel HTP) by an elutriation procedure. Long columns packed with small crystals were run under low pressure at acceptable flow rates, and were used over prolonged periods.

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