High resolution HPLC method with reverse phase C18 symmetry™ column for the analysis of tetracyclines produced byStreptomyces aureofaciensimmobilized in Ca-alginate gel
✍ Scribed by E. Gontier; M. L. Asanza Teruel; J. E. Nava Saucedo; J. N. Barbotin
- Book ID
- 104651701
- Publisher
- Springer-Verlag
- Year
- 1996
- Tongue
- English
- Weight
- 409 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-208X
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✦ Synopsis
To analyse the effect of immobilization of Streptomyces aureofuciens in Ca-alginate on tetracycline production, an HPLC technique for direct injection of the culture medium has been developed. The high resolution obtained with this method is achieved by using a Symmetry C18mreverse phase HPLC column. The comparison of our results with those previously described by different authors, shows that highest performances are obtained without using relatively drastic conditions such as 0.2 M oxalic acid at pH 2. Thus, our method, which has shown a very good reproducibility for hundreds of injections, is also better for life-time column preservation.