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High-resolution, high-throughput HLA genotyping by next-generation sequencing

✍ Scribed by G. Bentley; R. Higuchi; B. Hoglund; D. Goodridge; D. Sayer; E. A. Trachtenberg; H. A. Erlich


Book ID
110893065
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
795 KB
Volume
74
Category
Article
ISSN
0001-2815

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Mutation scanning using high-resolution melting curve analysis (HR-melt) is an effective and sensitive method to detect sequence variations. However, the presence of a common SNP within a mutation scanning amplicon may considerably complicate the interpretation of results and increase the number of