High resolution and high fields in biological solid state NMR
β Scribed by M Cotten; V.G Soghomonian; W Hu; T.A Cross
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 79 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0926-2040
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β¦ Synopsis
2H solid state NMR spectra of a polypeptide in an oriented membrane environment is demonstrated to have an orientational resolution of 0.3 degree. Such data results in high resolution structural constraints. Similar spectra are demonstrated at 23.2 T using a resistive magnet at the National High Magnetic Field Laboratory.
π SIMILAR VOLUMES
Many solid-state NMR experiments are sensitive to inhomogeneity in the radiofrequency field. We propose a method to restrict the sample volume, in magic angle spinning experiments, using a static magnetic field gradient and a selective pulse. The position of the gradient is calculated for our experi