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High-resolution 1H NMR spectroscopy in a live mouse subjected to 1.5 Hz magic angle spinning

โœ Scribed by Robert A. Wind; Jian Zhi Hu; Donald N. Rommereim

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
164 KB
Volume
50
Category
Article
ISSN
0740-3194

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โœฆ Synopsis

Abstract

It is demonstrated that the resolution of the ^1^H NMR metabolite spectrum in a live mouse can be significantly enhanced by an ultraslow magic angle spinning of the animal combined with a modified phaseโ€corrected magic angle turning (PHORMAT) pulse sequence. Proton NMR spectra were measured of the torso and the top part of the belly of a female BALBc mouse in a 2 T field while spinning the animal at a speed of 1.5 Hz. It was found that even in this relatively low field, with PHORMAT an isotropic spectrum is obtained with line widths that are a factor of 4.6 smaller than those obtained in a stationary mouse. It is concluded that in vivo PHORMAT has the potential to significantly increase the utility of ^1^H NMR spectroscopy for biochemical and biomedical animal research. Magn Reson Med 50:1113โ€“1119, 2003. ยฉ 2003 Wileyโ€Liss, Inc.

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