High prevalence of neutralizing activity to helicobacter pylori cytotoxin in serum of gastric-carcinoma patients
✍ Scribed by Masamichi Hirai; Takeshi Azuma; Shigeji Ito; Takuji Kato; Yoshihiro Kohli; Norio Fujiki
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- French
- Weight
- 808 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Helicobacter py/ori infection is causally related to chronic type-B gastritis, and may also be associated with an increased risk of gastric carcinoma. Vacuolating cytotoxin, which is an 87-kDa protein secreted by H. pylori, induces eukaryotic cell vacuolation in vitro. To determine whether there is an association between H. pylori vacuolating cytotoxin and gastric carcinoma, we investigated several characteristics of H. pylori infection, i.e, isolation of H. pylori from gastric biopsies, antibodies specific for H. pylori, detection of neuttalizing activity to vacuolating cytotoxin in serum and immunological detection of cytotoxin by serum. Out of 6 sera from gastric-carcinoma patients, all showed the neutralizing activity to vacuolating cytotoxin, in contrast to 3 of 5 sera from peptic-ulcer patients. Normal individuals showed no neutralizing activity. All sera possessing the neutralizing activity recognized an 87-kDa protein band by Western blot analysis. Our results confirmed that cytotoxinneutralizing activity in human sera was associated with immunodetection of an 87-kDa protein. To further evaluate neutralizing activii in serum from gastric-carcinoma patients, we retrospectively analyzed frozen-stocked serum samples from 22 gastriccarcinoma patients. Sera from 2 I of these 22 patients exhibited neutralizing activity. These sera were also checked for antibodies to H. pylori, using an ELISA; I 6 sera showed positive results. Our results indicate that detection of cytotoxin-neutralizing activity in sera is strongly associated with H. pylori infection, and probably with gastric carcinoma, and is also of interest in the diagnosis of H. pylori infection.