High pressure study of fluorescence polarization in plyacrylamide
β Scribed by G. Chryssomallis; H.G. Drickamer
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 172 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0009-2614
No coin nor oath required. For personal study only.
β¦ Synopsis
The fluorescence polarization behavior of a series of polyacrylamide-fluorescein conjugates in dilute aqueous solution was studied by lowering the temperature, and by isothermally increasing the pressure. The data are consistent with the previously proposed explanation that the relaxation process is the cooperative motion of a few monomeric units at the chain end.
π SIMILAR VOLUMES
High pressure liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA) were compared in a quinidine pharmacokinetic study. Six healthy male subjects received single oral doses of regular release (RR) quinidine sulfate, sustained release (SR) quinidine bisulfate and the same dose
The binding of proflavine to L)NA has been studied by measuring the polarization and intensity of emission of DNA-dye complexes. Such measurements also permit the determination of the fluorescence of the bound dye as a function of the degree of binding. Techniques of emission spectroscopy permit the
The liquid crystal orientation technique has been utiiized to determine the j#arizatfon of the emission from complexes formed in the excited state. The experimentaL results agree wtth theoretfcal predicttons made for hetero-excimers with appreciable admixture of locally excited states.
We have studied aggregation/association of monodisperse DNA fragments (ranging from 30-90 base pairs) by steady-state fluorescence polarization of intercalculated ethidium. The method of excitation a t different wavelengths in the ethidium absorption spectrum provides information about anisotropic t
Raman spectra of pressure-induced crystalline CHzBrz have been measured at room temperature for pressures up to 240 kbar in a gasketed diamond anvil cell. The pressure dependence of the internal and lattice modes are reported and discussed. A phase transition appears between 170 and 240 kbar. This h