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High Pressure Liquid Chromatography Determination of Histamine in Fish

โœ Scribed by J. P. GOUYGOU; C. SINQUIN; P. DURAND

Book ID
108812229
Publisher
Institute of Food Technologists
Year
1987
Tongue
English
Weight
331 KB
Volume
52
Category
Article
ISSN
0022-1147

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr