High-pressure liquid chromatographic determination of ftorafur [1-(tetrahydro-2-furanyl)-5-fluorouracil] and GLC-mass spectrometric determination of 5-fluorouracil and uracil in biological materials after oral administration of uracil plus ftorafur
✍ Scribed by Teruyoshi Marunaka; Yukihiko Umeno; Kozo Yoshida; Masahiro Nagamachi; Yoshinori Minami; Setsuro Fujii
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 591 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
✦ Synopsis
Oral adminstration of uracil plus ftorafur [ 1-(tetrahydro-2-furanyl)-5-fluorouracil] has a greater antitumor effect than that of ftorafur alone. A high-pressure liquid chromatographic method was developed for the determination of ftorafur in plasma and visceral tissues with a sensitivity of 0.025 lg/ml or g of wet weight. A combination of GLC-mass fragmentography and GLC-mass spectrometry with total-ion monitoring was developed for the specific, simultaneous determination of 5-fluorouracil, an active metabolite, and uracil as their trimethylsilylated derivatives. The detection limits for 5-fluorouracil and uracil in the first method were 0.001 pg/ml for plasma and 0.001-0.005 fig/ml for visceral tissues, and those in the second method were 0.2 pg/ml or g of wet weight. The precision and sensitivity of the assay appear to be satisfactory for determination of the levels of these compounds in plasma and visceral tissues.
Keyphrases 0 Ftorafur-high-pressure liquid chromatographic analysis in biological materials 0 5-Fluorouracil-GLC-mass spectrometric analysis in biological materials 0 Uracil-GLC-mass spectrometric analysis in biological materials 0 High-pressure liquid chromatography-analysis, ftorafur in biological materials GLC-mass spectrometry-analysis, 5-fluorouracil and uracil in biological materials Antineoplastic agents-uracil plus ftorafur, high-pressure liquid chromatographic and GLC-mass spectrometric analysis in biological materials