High-pressure liquid chromatographic analysis of isomeric aminophenols with electrochemical detection

✍ Scribed by Larry A. Sternson; Wayne J. DeWitte

• Book ID: 104145297
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 217 KB
• Volume: 138
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98016-6

✦ Synopsis

In a previous report', we described the high-pressure liquid chromatographic (HPLC) separation and quantitation of isomeric hydroxyanilines involving reversedphase partition chromatography of the mixtures on a C,, column with the mobile phase methanol-water (15%) containing 0.26 M ammonium acetate (pH 7) and 0.015 M nickel acetate. Spectrophotometric monitoring of the-effluent at 254 nm permitted quantitation to co. 5. lo-" M in aminophenol. Sensitivity was limited by the molar absorptivity of the aminophenols (a,,, M 103; eoorlho m 3'00) at 254 nm. In this report, the same chromatographic system is used, but advantage is taken of the electrochemical oxidizability of these compounds to provide a more sensitive detection of the aminophenols after HPLC separation, capable of quantitating nanomolar levels.