High-pressure infrared spectroscopic evidence of water binding sites in 1,2-diacyl phospholipids
✍ Scribed by P.T.T. Wong; H.H. Mantsch
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 823 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0009-3084
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✦ Synopsis
The sites of water binding in L-l,2-diacylphosphatidylcholines (L-DPPC) were investigated by infrared spectroscopic methods. Evidence is provided that water molecules in fully hydrated samples, and the crystallization water in solid samples, are hydrogenbonded to the phosphate oxygen atoms of the PO~ group in the head moiety, as well as to the ester C----O group of the sn-2 acyl chain in the interfacial region. The results indicate that no hydrogen-bonding occurs between water molecules and the ester C=O group of the sn-I acyl chain of L-l,2-dipaimitoylphosphatidylethanolamine (L-DPPE), L-DPPC and L-l,2-dimyristoylphosphatidylcholine (L-DMPC). Although there is no hydrogen-bonding between water and the choline moiety, water molecules are attached to the (CH 3)3N ÷ group by polar interactions.