High-pressure Freezing in CLEM : A Correlative Light Electron Microscopy Study

✍ Scribed by Paul Verkade

• Publisher: Wiley (John Wiley & Sons)
• Year: 2007
• Weight: 351 KB
• Volume: 9
• Category: Article
• ISSN: 1439-4243
• DOI: 10.1002/imic.200790123

✦ Synopsis

Research methods using so-called Correlative Light Electron Microscopy (CLEM) techniques have gained increasing attention in recent years. Most techniques have focused on the light microscopy part of the process. The processing for electron microscopy, however, deserves attention as well to get the most out of the technique. Some new developments, including the use of a superior fixation technique, are described. periment. The sample is then fixed, immuno labeled for GFP and the immuno label visualized as a dark electron dense precipitate (DAB) [4]. Grabenbauer et al.

[5] recently succeeded in avoiding the immuno labeling part by using photo-