๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

High Pressure Fosters Protein Refolding from Aggregates at High Concentrations

โœ Scribed by Richard J. St. John, John F. Carpenter and Theodore W. Randolph

Book ID
123642265
Publisher
National Academy of Sciences
Year
1999
Tongue
English
Weight
913 KB
Volume
96
Category
Article
ISSN
0027-8424

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Protein Refolding at High Concentrations
Protein Refolding at High Concentrations Using Detergent/Phospholipid Mixtures
โœ G. Zardeneta; P.M. Horowitz ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 650 KB

Refolding of the sulfurtransferase enzyme rhodanese (EC 2.8.1.1) at high concentrations (0.2 mg/ml) was obtained at significant yields (> or = 45%) by using mixtures composed of detergents (either Triton X-100 or lauryl maltoside) and phospholipids. It is presumed that the ratio of detergent to phos

Protein refolding at high concentration
Protein refolding at high concentration using size-exclusion chromatography
โœ Boris Batas; Julian B. Chaudhuri ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 809 KB

A new method to improve refolding yields and t o increase the concentration of refolded proteins in a single operation has been developed. The method uses size-exclusion chromatography matrices to perform buffer exchange, aggregate removal, and the folding reaction. The reduced diffusion of proteins