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High-Performance Thin-Layer Chromatographic analysis of hepatoprotective diterpenoids from Andrographis paniculata

✍ Scribed by Sudhanshu Saxena; Dharam C. Jain; Madan M. Gupta; Rajendra S. Bhakuni; Hari O. Mishra; Ram P. Sharma

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 51 KB
Volume: 11
Category: Article
ISSN: 0958-0344
DOI: 10.1002/(sici)1099-1565(200001/02)11:1<34::aid-pca487>3.0.co;2-v

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✦ Synopsis

A rapid and simple high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitative estimation of the biologically active diterpenoids, 14-deoxy-11,12didehydroandrographolide, andrographolide, neoandrographolide and andrographiside in Andrographis paniculata. The assay combines the isolation and separation of andrographolide derivatives on silica gel 60 F 254 HPTLC plates with spot visualization and scanning at 540 nm. Methanol was found to be the most appropriate solvent for the exhaustive extraction of andrographolide derivatives.

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