High-performance size-exclusion chromatography of hydrolyzed plant proteins

✍ Scribed by Howard G. Barth

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 764 KB
• Volume: 124
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90237-8

✦ Synopsis

A high-performance size-exclusion chromatographic (SEC) procedure has been developed to determine the molecular size distribution of several sources of hydrolyzed plant proteins including soy, potato, cotton seed, corn gluten, wheat bran, and wheat germ. The SEC packing consisted of a glycerylpropylsilyl layer covalently bonded to 100-A pore-size silica particles (10 pm). A number of mobile phases were evaluated in an attempt to reduce adsorption between hydrolyzed components and packing material. Systems containing either sodium dodecyl sulfate or methanol showed the best performances; however, adsorption could not totally be eliminated. The combination of size-exclusion and retention mechanisms of separation was valuable in establishing differences among samples. Based on dialysis of hydrolyzed soy protein, it appeared that most of the adsorbed material was of low molecular weight. Column recovery studies indicated complete elution of samples. * This paper was presented at the International Symposium on HPLC of Proteins and Peptides,