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High-performance liquid chromatography study of water-soluble ternary polyacrylamide-metal-protein complexes

✍ Scribed by Betül Dinçer; M. I. Mustafaev; Seval Bayülken

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 121 KB
Volume: 65
Category: Article
ISSN: 0021-8995
DOI: 10.1002/(sici)1097-4628(19970705)65:1<37::aid-app5>3.0.co;2-r

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✦ Synopsis

Lethal or harmful effects of X-rays and gamma rays are known to be reduced by prior administration of certain ''radioprotector'' chemicals. In this work, complex formation of polyacrylamide with bovine serum albumin (as a model protein) in the presence of divalent copper ions (Cu 2/ ) was investigated with high-performance liquid chromatography (HPLC) before evaluating its possible use as a radioprotector. HPLC results determined the most suitable metal concentration and protein/polymer ratio for maximum complex formation between polymer and protein molecules.

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