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High-performance liquid chromatography of some AMP derivatives used as affinity ligands

✍ Scribed by J. Kovář; V. Kahle; J. Turánek

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
495 KB
Volume
258
Category
Article
ISSN
1873-3778

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✦ Synopsis

Separation

of AMP, N'-carboxymethyl-AMP, N6-carboxymethyl-AMP and N6-[N-(6aminohexyl)carbamoylmethyl]-AMP by reversed-phase liquid chromatography is described. A 25 mM sodium phosphate buffer (pH 2.555.0) proved to be the most suitable mobile phase for the separation of AMP and the intermediates, while for the elution of the final product 10 % methanol in the buffer (pH 2.5) gave satisfactory results. Identification of the peaks was performed by means of ultraviolet and infrared spectroscopic measurements, by cyclic voltammetry and by reaction with ninhydrin.

Analysis of the reaction mixtures at successive stages of the synthesis of N6-[N-(6aminohexyl)carbamoylmethyl]-AMP by high-performance liquid chromatography enabled us to propose two modifications of the separation procedure of intermediates and final product.

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