✦ LIBER ✦

High-performance liquid chromatography of fatty acid isopropylidene hydrazides and its application in lipid analysis

✍ Scribed by Vishwanath P. Agrawal; Erhard Schulte

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 242 KB
Volume: 131
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90183-5

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✦ Synopsis

Fatty acid isopropylidene hydrazides, prepared by stepwise treatment of acyl lipids with hydrazine and acetone, were analyzed by high-performance liquid chromatography on a reversed-phase column. These derivatives could be easily eluted with 15% water in methanol and monitored by measuring absorbance at 229 nm with a uv detector. Their elution behavior, in general, was similar to that of methyl esters and some commonly used ultraviolet-absorbing derivatives of fatty acids. The new method has been used for fatty acid analysis of some oils.

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