High-performance liquid chromatography of amino acids, peptides and proteins : LXXXV. Separation of isoforms of the glycoprotein hormones from human pituitary extracts

✍ Scribed by Robyn C. Johnston; Peter G. Stanton; David M. Robertson; Milton T.W. Hearn

• Book ID: 104145840
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 682 KB
• Volume: 397
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85023-8

✦ Synopsis

Procedures for the high-resolution separation of microheterogeneous forms of the glycoprotein hormones, lutropin, follitropin and thyrotropin, present in human pituitary extracts are described. The sequential use of preparative isoelectric focusing and ion-exchange high-performance liquid chromatography (HPLC) allows the further discrimination of the isoforms of these glycoprotein hormones, previously considered homogeneous on the basis of their electrophoretic mobility. The ability of ion-exchange HPLC stationary phases to probe for slight differences in charge asymmetry expressed on the surface of these proteins raises the possibility of their rapid purification via the tandem on-line use of the same ion-exchange support with several different elution systems. Retention maps, illustrating the basis of this approach, are described.