After precipitation of proteins; serum, hepatocytes, or glutathione-derivatized bovine serum albumin, by perchloric acid, dithiothreitol was used to reduce glutathione-protein mixed disulfides in the ether-washed, resuspended pellet. Following neutralization and Scarboxymethylation of free s&hydra1
High-Performance Liquid Chromatography Method for the Determination of Protein-Glutathione Mixed Disulfide
โ Scribed by K. Kumari; P. Khanna; N.H. Ansari; S.K. Srivastava
- Book ID
- 118768026
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 232 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
Glutathione (GSH) and glutathione disulfide (GSSG) are biologically important intracellular thiols; alterations in the GSH/GSSG ratio are often used to assess exposure of cells to oxidative stress. Although several methods are available for measuring GSH and GSSG, all have some disadvantages includi
A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 related sulfur-containing amino acids or their derivatives has been described. The procedure is based upon the i