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High-performance liquid chromatography for assaying NAD glycohydrolase from Neurospora crassa conidia

โœ Scribed by Piergiorgio Pietta; Mario Pace; Faustino Menegus

Book ID
102629675
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
320 KB
Volume
131
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A rapid and sensitive high-performance liquid chromatographic technique was developed to determinate NAD glycohydrolase (EC 3.2.2.5.) activity from Neurospora crassa conidia. The separation of the assay substrate and products was achieved by isocratic reverse-phase chromatography and the peaks were detected by the absorbance at 259 nm. Quantities of NAD+ and nicotinamide as small as 10 pmol could be measured.

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