High-performance liquid chromatography determination of nitrated polycyclic aromatic hydrocarbons by indirect fluorescence detection

✍ Scribed by Salma M. Al-Kindy; James N. Miller

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 112 KB
• Volume: 23
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1098

✦ Synopsis

Abstract

A high‐performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre‐concentrated using solid‐phase extraction and well resolved on a C~18~ column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8‐tetrahydronaphthol (5,6,7,8‐THN‐1‐OH), 7‐amino‐4‐methyl coumarin (Coumarin 120, COU‐120) and 3‐hydroxy‐4‐(2‐hydroxy‐4‐sulfo‐1‐naphthylazo)2‐naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 × 10^−9^ to 1.1 × 10^−8^ mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 × 10^−10^ mol/L for 1‐nitronaphthalene and 2.1 × 10^−11^ mol/L for 2‐nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100%. Copyright © 2008 John Wiley & Sons, Ltd.