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High-performance liquid chromatography and ultraviolet spectrophotometry for quantitation of tryptophan in barytic hydrolysates

โœ Scribed by S. Delhaye; J. Landry

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
254 KB
Volume
159
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A procedure for quantitation of tryptophan in feedstuffs is described. It is based on barytic hydrolysis of material at 125ยฐC for 16 h, acidification of hydrolysate to pH 3 with HCl, highperformance liquid chromatography on Nova Pak C,s (Waters Assoc.), and spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme added to samples ranges from 98.7 to 100%.

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