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High Performance Liquid Chromatography and Photodiode Array Detection of Phenylpropanoids and Benzoates in Rubus Protoplasts Elicited by Kinetin

✍ Scribed by Lionel Chevolot; Robin Dubois-Dauphin; Jean Claude Yvin; Yvette Liénart

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
127 KB
Volume
8
Category
Article
ISSN
0958-0344

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✦ Synopsis

Reverse-phase high performance liquid chromatography with photodiode array detection (PAD) has been developed for simultaneous separation, identification and quantification of picomoles of phenylalanine ammonialyase (PAL) reaction products. Separation has been achieved in 30 min on a reverse-phase silica column using methanol:water gradient elution, and PAD provided sensitive and specific determination of phenolics. The method has been applied to biological samples to study kinetin-induced changes in phenol pathways within protoplasts of Rubus fruticosus (raspberry).

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