High Performance Liquid Chromatography

✍ Scribed by Heinz Engelhardt (auth.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1979
• Tongue: English
• Leaves: 262
• Series: Chemical Laboratory Practice
• Edition: 1
• Category: Library

✦ Synopsis

Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta­ cular development in analytical chemistry and has not yet abated be­ cause its vast potential for application remains to be fully exploit­ ed. Significant factors contributing to this continued rise are the simplicity and low cost of the required basic equipment and the rela­ tive ease of acquiring and interpreting the data. Unfortunately, in LC, as so often in the field of analytical chemistry, the available commercial instruments are frequently far more complicated - and consequently far more expensive - than is nec­ essary for routine application. Therein also lies the risk of propa­ gating a "black box" philosophy that would be particularly detrimen­ tal to chromatography. Moreover, it appears to have been forgotten, as was done previously with gas chromatography, that inadequate sep­ aration by a column can be remedied only with great difficulty, if at all, by electronic means. Also, whether the capillary columns recent­ ly advocated with great enthusiasm for LC will fulfill the expecta­ tions of their proponents is highly questionable unless someone comes up with some new and revolutionary ideas.

✦ Table of Contents

Front Matter....Pages I-XII
Chromatographic Processes....Pages 1-5
Fundamentals of Chromatography....Pages 6-31
Equipment for HPCL....Pages 32-57
Detectors....Pages 58-80
Stationary Phases....Pages 81-95
Adsorption Chromatography....Pages 96-163
Partition Chromatography....Pages 164-188
Ion-Exchange Chromatography....Pages 189-205
Exclusion Chromatography Gel Permeation Chromatography....Pages 206-221
Selection of the Separation System....Pages 222-230
Special Techniques....Pages 231-239
Purification of Solvents....Pages 240-242
Back Matter....Pages 243-250

✦ Subjects

Analytical Chemistry; Ecotoxicology; Biochemistry, general