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High-performance liquid chromatographic separation of unusual amino acid enantiomers derivatized with (1S,2S)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl-isothiocyanate

✍ Scribed by A. Péter; M. Péter; F. Fülöp; G. Török; G. Tóth; D. Tourwé; J. Sápi


Book ID
105594617
Publisher
Springer
Year
2000
Tongue
English
Weight
703 KB
Volume
51
Category
Article
ISSN
0009-5893

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✍ Yoshihiko Watanabe; Kazuhiro Imai 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 139 KB

7-Fluoro+nitrobenzo-2-oxa-I .3-diazole is used as a precolumn fluorescent labeling reagent for high-performance liquid chromatography of amino acids. including proline and hydroxyproline. The reaction is run at pH 8.0 at 60°C for 5 min. The fluorophors (Asp, GIL Hyp. Ser, Gly. Thr, Ala, Pro) are se