High-performance liquid chromatographic separation of linear and cyclic condensed phosphates
โ Scribed by Hisashi Yamaguchi; Terumasa Nakamura; Yukio Hirai; Shigeru Ohashi
- Book ID
- 108335271
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 740 KB
- Volume
- 172
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
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We developed a HPLC method which separates the following nine inositol-containing compounds of biological interest: inositol, inositol 1-monophosphate, inositol 2- or 4-monophosphate, inositol 1,2-cyclic phosphate, inositol 1,4-bisphosphate, inositol 1,4,5-trisphosphate, glycerophosphoinositol, glyc
Separation of inositol phosphates by low-pressure anion-exchange chromatography yields unsatisfactory results, while previously described anion-exchange HPLC methods require such extensive processing times that they preclude efficient sample analysis. Using a low-capacity Vydac nucleotide anion-exch