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High-performance liquid chromatographic separation of detritylated oligonucleotides on highly cross-linked poly-(styrene-divinylbenzene) particles

✍ Scribed by Christian G. Huber; Peter J. Oefner; Günther K. Bonn

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
315 KB
Volume
599
Category
Article
ISSN
1873-3778

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## Abstract The LC performance of a 1×50 mm polymer monolithic column format was demonstrated with high‐peak capacity one‐ (1D) and offline two dimensional (2D) LC separations of intact proteins. After optimizing the RP 1D‐LC conditions, including column temperature, flow rate and gradient time, a