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High-performance liquid chromatographic measurement of guanidino compounds of clinical importance in human urine and serum by pre-column fluorescence derivatization using benzoin

✍ Scribed by Masaaki Kai; Takashi Miyazaki; Yosuke Ohkura

Book ID
108449894
Publisher
Elsevier Science
Year
1984
Weight
688 KB
Volume
311
Category
Article
ISSN
0378-4347

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Improved high-performance liquid chromat
Improved high-performance liquid chromatographic determination of guanidino compounds by pre-column dervatization with ninhydrin and fluorescence detection
✍ Wolfgang Buchberger; Matthias Ferdig πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 297 KB

## Abstract Ninhydrin has been investigated as a pre‐column derivatization reagent for guanidino compounds. The reaction takes place under strongly alkaline conditions, followed by a second step at low pH and elevated temperature. This procedure yields derivatives with favourable fluorescence prope