High-performance liquid chromatographic enantioseparations on capillary columns containing monolithic silica modified with cellulose tris(3,5-dimethylphenylcarbamate)

✍ Scribed by Bezhan Chankvetadze; Chiyo Yamamoto; Nobuo Tanaka; Kazuki Nakanishi; Yoshio Okamoto

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 196 KB
• Volume: 27
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200401819

✦ Synopsis

Abstract

Monolithic capillary columns containing native silica gel were modified with cellulose tris(3,5‐dimethylphenylcarbamate) (CDMPC) and used for enantioseparations in capillary liquid chromatography. The method adopted for in situ enantioselective modification of monolithic fused silica capillary columns by coating with CDPMC appears to be fairly simple and fast. High efficiency enantioseparations of test racemic compounds and several chiral drugs were achieved in a short time. It was possible to increase the amount of chiral selector present by multiple coating of monoliths with CDMPC. The baseline enantioseparation of 2,2,2‐trifluoro‐1‐(9‐anthryl)ethanol was achieved in an analysis time less than 30 s with this capillary column. In addition, reproducible enantioseparations were obtained when the chiral selector was removed from the monolithic column by flushing it with appropriate solvent and the column recoated.