High-performance liquid chromatographic determination of vincristine sulfate in preformulation studies

✍ Scribed by J. E. Bodnar; J. R. Chen; W. H. Johns; E. P. Mariani; E. C. Shinal

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 333 KB
• Volume: 72
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600720514

✦ Synopsis

A fast and simple procedure was developed for the quantitative determination of vincristine sulfate for use in preformulation studies. The procedure involves the use of high-performance liquid chromatography with a reverse-phase column and a mobile phase containing the sodium salt of 1-pentanesulfonic acid for ion-pairing. The procedure has een shown to be specific for vincristine sulfate in the presence of forced degradation products of this substance, vinblastine (a structurally similar Vinca alkaloid), and several possible formula excipients. The procedure is linear from 10-200% of the normal injection concentration, and has an assay precision (relative 2u) of 61.6%. Recovery of known samples averaged 99.7%.

Keyphrases Vincristine sulfate-high-performance liquid chromatography, preformulation studies, degradation 0 High-performance liquid chromatography-vincristine sulfate, degradation products 0 Preformulation studies-vincristine sulfate, degradation products, high-performance liquid chromatography mg) was accurately weighed and transferred to a 100-ml volumetric flask. ~ ~~~~~ p-Bondapak and p-Porasil Liquid Chromatography Columns Care and Use Burdick and Jackson Laboratories, Inc., Muskegon, Mich. Type HA, 0 . 4 5 ~ filter, Millipore Corp., Bedford, Mass.