High-performance liquid chromatographic determination of prophylthiouracil in plasma

✍ Scribed by M.T. Rosseel; R.A. Lefebvre

• Book ID: 104145616
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 274 KB
• Volume: 507
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84201-1

✦ Synopsis

A high-performance liquid chromatographic method for the determination of the antithyroid drug propylthiouracil in dog plasma has been developed. Propylthiouracil and the internal standard, methylthiouracil, are extracted from plasma with methylene chloride at pH 6 and the organic layer is evaporated to dryness. The residue is chromatographed on a Chromspher C rs reversed-phase column using Pit B-7 (0.005 M 1-heptanesulphonic acid in water)-1 % acetic acid-methanol (40:45: 15, v/v/v) as the mobile phase. Quantification is achieved by monitoring the UV absorbance at 300 nm. The response is linear (0.1-15 pg/ml) and using 100 ~1 of plasma the detection limit is 50 ng/ml. The within-run coefficient of variation is < 5% and the accuracy is within 10% of the theoretical value at concentrations between 0.1 and 15 pg/ml plasma.