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High-performance liquid chromatographic determination of native and reduced α-glucan oligosaccharides on reversed phase columns using post-column immobilized enzyme reactors

✍ Scribed by P. C. Maes; L. J. Nagels; B. R. Spanoghe

Book ID
112723002
Publisher
Springer
Year
1993
Tongue
English
Weight
581 KB
Volume
37
Category
Article
ISSN
0009-5893

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High-performance liquid chromatographic
High-performance liquid chromatographic determination of glucosides (glucose conjugates) with post-column reaction detection combining immobilized enzyme reactors and luminol chemiluminescence
✍ Philip J. Koerner Jr.; Timothy A. Nieman 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 900 KB

This detection method makes use of sequential immobilized enzyme reactors (IMER) to first hydrolyze D-D-glucosides to /&D-glucose (using fi-glucosidase) and then to produce hydrogen peroxide from the P-o-glucose (using glucose oxidase); the hydrogen peroxide is then detected with luminol chemilumine