High-performance liquid chromatographic determination of kanamycin

✍ Scribed by David L. Mays; Richard J. Van Apeldoorn; Ronald G. Lauback

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 715 KB
• Volume: 120
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)99000-4

✦ Synopsis

A fast, selective, and precise liquid chromatographic method for simultaneous, independent determination of kanamycins A and B is described. Sample components are separated on a pellicular cation exchanger and monitored by fluorescence using post-column on-line derivatization. Less than 0.35 /xg of kanamycin B can be detected in as much as 7 pg kanamycin A injected. The detection limit for kanamycin A is less than 20 ng injected. Reproducibility of the entire chromatographic system is about 1% (2 G) based upon repeated injections of standards. Precision of repeated process sample preparations is about 6% (2 0). Chromatographic analysis time is less than 15 min per sample.