High-performance liquid chromatographic determination of histamine in biological samples after derivation with o-phthalaldehyde
✍ Scribed by A.L. Rönnberg; C. Hansson; R. Håkanson
- Book ID
- 107711968
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 536 KB
- Volume
- 139
- Category
- Article
- ISSN
- 0003-2697
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A reversed-phase high-performance liquid chromatographic method for the detection of boronophenylalanine is described. Determination was obtained by precolumn reaction of o-phthalaldehyde with a mixture of standard amino acids containing boronophenylalanine and separating the corresponding o-phthala
Based on studies of the reaction between histamine and o-phthalaldehyde in alkaline solution, a method optimized for the determination of histamine in biological samples by means of HPLC and postcolumn o-phthalaldehyde derivatization has been developed. The method permits determination of histamine