High-performance liquid chromatographic determination of dicloxacillin in presence of its degradation products

✍ Scribed by G. Lauriault; D.V.C. Awang; D. Kindack

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 258 KB
• Volume: 283
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96290-3

✦ Synopsis

Recently the need for a rapid, reliable method for determination of cloxacillin formulations led to the development of a high-performance liquid chromatographic (HPLC) assay procedure', which gave results that were in excellent agreement with those obtained by the British Pharmacopoeia (BP) calorimetric method*.

As predicted by the authors l, the HPLC method is directly and satisfactorily applicable to the analysis of oxacillin in the presence of its degradation products, but, contrary to their expectations, the method required adjustment for detection of the major product of acid degradation of dicloxacillin*, which was not detected under the original conditions. The procedure herein reported thus allows determination of dicloxacillin in the presence of its products of degradation in both basic and acid media.

EXPERIMENTAL

An SP 8000 liquid chromatograph (Spectra-Physics, Santa Clara, CA, U.S.A.), in conjunction with an SP 8440 variable-wavelength detector and a reversed-phase column (RP-2, 10 pm, 25 cm x 4.6 mm I.D.