High-performance liquid chromatographic determination of dialkyltin homologues using fluorescence detection

✍ Scribed by Tai-Hong Yu; Yasuaki Arakawa

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 534 KB
• Volume: 258
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96411-2

✦ Synopsis

A simple, sensitive and reproducible method has been developed for the simultaneous determination of dialkyltin compounds and their possible metabolites. Dialkyltin compounds were separated by high-performance liquid chromatography on a cyanopropyl-bonded column with n-hexaneethyl acetate (955) containing 5% acetic acid and detected by a fluorescence detector with an excitation wavelength at cu. 420 nm and an emission wavelength at cu. 500 nm after post-column derivatization with Morin reagent. Detection limits ranged from 0.1 to 1 ng depending upon the dialkyltin species. Recoveries of dialkyltins added to various tissues at the 5nmole level ranged from 91 to 99%.