High-performance liquid chromatographic assay for the antiprotozoal agent, tinidazole, in human plasma

✍ Scribed by K. B. Alton; J. E. Patrick

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 321 KB
• Volume: 68
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600680522

✦ Synopsis

A rapid and specific high-performance liquid chromatographic (HPLC) assay for the quantitative determination of tinidazole in human plasma is described. After a simple extraction procedure, the compound is analyzed by HPLC using a reversed-phase column and a UV detector. Quantitation is accomplished using an external standard, and peak areas are determined with an integrating computer. The average recovery of tinidazole over a concentration range of 0.20-20.0 microgram/ml was 86.9 +/- 2.8% SD. The maximum sensitivity of the assay is approximately 0.10 microgram/ml; it is suitable for use in pharmacokinetic studies following administration of therapeutic levels of tinidazole to humans.