High-performance liquid chromatographic analysis of oligosaccharides and glycopeptides accumulating in lysosomal storage disorders

✍ Scribed by N.M.K. Ng Ying Kin; L.S. Wolfe

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 529 KB
• Volume: 102
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90341-3

✦ Synopsis

A high-performance liquid chromatography method was developed to separate the oligosaccharides and glycopeptides which accumulate in the liver and are excreted in the urine of a number of lysosomal storage disorders. Characteristic elution profiles were obtained for oligosaccharides, their borohydride reduced products, and glycopeptides in mannosidosis. fucosidosis, G,,,,-gangliosidosis, G,,-gangliosidosis variant 0, sialidosis, and aspartylglucosaminuria. Chromatographic separations could be completed in 20 min.