High-performance liquid chromatographic analysis of methadone hydrochloride oral solution

✍ Scribed by Thomas H. Beasley Sr.; Howard W. Ziegler

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 374 KB
• Volume: 66
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600661224

✦ Synopsis

A direct and rapid high-performance liquid chromatographic assay for methadone hydrochloride in a flavored oral solution dosage form is described. A syrup sample, one part diluted with three parts of water, is introduced onto a column packed with octadecylsilane bonded on 10 micrometer porous silica gel (reversed phase). A formic acid-ammonium formate-buffered mobile phase is linear programmed with acetonitrile. The absorbance is monitored continuously at 280 or 254 nm, using a flow-through, UV, double-beam photometer. An aqueous methadone hydrochloride solution is used for external standardization. The relative standard deviation was not more than 1.0%. Drug recovery from a syrup base was better than 99.8%.